Detecção dos subtipos do papilomavírus humano em lesões precursoras do câncer do colo uterino em mulheres jovens

Abstract

Introduction: Cervical cancer is the third most prevalent type of cancer in Brazil and the first in the State of Pará. It is caused by HPV, which has more than 200 subtypes, and its precursor lesion is differentiated into low and high grade. Objective: to detect the most prevalent HPV subtypes in precursor lesions of cervical cancer in young women. Method: The samples come from a reference laboratory in Belém and consist of 27 biopsies of patients, who had positive PCR for HPV, divided into 3 groups, with 9 samples from patients with low-grade intraepithelial lesions, 3 samples from high-grade intraepithelial lesions and 7 samples with cervicitis used as a control group. The samples had the DNA isolated with a DNA extraction kit for paraffined block. Nested-PCR were performed to detect the presence of HPV DNA, and positive samples were subjected to real-time PCR for 7 probes of the following viral subtypes: 6, 11, 16, 18, 31, 33, 35, 52 and 58 Result: A total of 19 samples were HPV DNA positive. The prevalence of LIEBG was higher than that of LIEAG. The most prevalent HPV subtype was HPV 16, with a total of 3 samples in relation to the total subtypes. No HPV DNA 18, 31, 58 and 11 were found in any of the samples. Conclusion: The prevalence of HPV in young women was 70,3%. In women with cervicitis, the prevalence was 58,3%, in LIEBG it was 81,8% and in LIEAG 75%. The most prevalent HPV subtype was 16, with 15.7% of the total samples. Regarding cervicitis, the most prevalent subtypes were HPV 33 and HPV 52. In LIEBG there was no detection of any subtype tested in the samples. In LIEAG the most prevalent subtype was HPV 16, followed by HPV 35.