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Item Detecção do HPV nas lesões pré-câncer de colo uterino em laboratório na região Amazônica(Centro Universitário do Estado do Pará, 2022-11-14) Alves, Emanuelle Ferreira; Magalhães, Marília Alvino de; Chagas, Elcimara da Paixão Ferreira; http://lattes.cnpq.br/4061268902131720; Rodrigues, Paloma Panzuti; http://lattes.cnpq.br/9380681051150645; Dutra, Taísa Andrade Pereira; http://lattes.cnpq.br/4788667938871523Cervical cancer is the fourth most common among the Brazilian female population and, in global terms, it also ranks among the top positions. In 99% of cases, this cancer is caused by HPV infection, with genotypes 16 and 18 being the most involved. To identify the most common HPV genotypes in lesions classified as LIEBG and LIEAG. Samples of the uterine cervix were collected and submitted to cytopathological analysis, and those diagnosed with LIEBG or LIEAG had their DNA extracted and amplified. For HPV detection, the “Nested-PCR” technique was used with oligonucleotide primers MY9/MY11, and also GP5 and GP6; and for 6, 11, 16, 18, 31, 33, 35, 52 and 58 subtypes typing, real-time PCR was performed with specific probes. HPV was present in 45 (90%) of the 50 patients with LIEBG or LIEAG. In the 23 patients who had LIEBG and positive HPV DNA, the most common genotype was HPV 58 (34.78%). On the other hand, in the 22 patients with LIEAG and positive HPV DNA, the most common genotype was HPV 16 (50%). Disregarding the degree of intraepithelial lesion present, HPV 16 was the most common among the 45 women with positive HPV DNA. HPV infection was detected in most patients with squamous intraepithelial lesion, with types 16 and 58 being the most common. The virus genotypes found were similar between LIEBG and LIEAG, however HPV 16 had a prevalence twice as high in high-grade lesions in relation to low-grade lesions.Item Detecção dos subtipos do papilomavírus humano em lesões precursoras do câncer do colo uterino em mulheres jovens(Centro Universitário do Estado do Pará, 2020) Elias, Carina de Abreu; Baia, Rafael Miranda Pantoja; Chagas, Elcimara da Paixão Ferreira; http://lattes.cnpq.br/4061268902131720Introduction: Cervical cancer is the third most prevalent type of cancer in Brazil and the first in the State of Pará. It is caused by HPV, which has more than 200 subtypes, and its precursor lesion is differentiated into low and high grade. Objective: to detect the most prevalent HPV subtypes in precursor lesions of cervical cancer in young women. Method: The samples come from a reference laboratory in Belém and consist of 27 biopsies of patients, who had positive PCR for HPV, divided into 3 groups, with 9 samples from patients with low-grade intraepithelial lesions, 3 samples from high-grade intraepithelial lesions and 7 samples with cervicitis used as a control group. The samples had the DNA isolated with a DNA extraction kit for paraffined block. Nested-PCR were performed to detect the presence of HPV DNA, and positive samples were subjected to real-time PCR for 7 probes of the following viral subtypes: 6, 11, 16, 18, 31, 33, 35, 52 and 58 Result: A total of 19 samples were HPV DNA positive. The prevalence of LIEBG was higher than that of LIEAG. The most prevalent HPV subtype was HPV 16, with a total of 3 samples in relation to the total subtypes. No HPV DNA 18, 31, 58 and 11 were found in any of the samples. Conclusion: The prevalence of HPV in young women was 70,3%. In women with cervicitis, the prevalence was 58,3%, in LIEBG it was 81,8% and in LIEAG 75%. The most prevalent HPV subtype was 16, with 15.7% of the total samples. Regarding cervicitis, the most prevalent subtypes were HPV 33 and HPV 52. In LIEBG there was no detection of any subtype tested in the samples. In LIEAG the most prevalent subtype was HPV 16, followed by HPV 35.