Medicina

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Navegando Medicina por Orientador "Chaves, Rosa Helena de Figueiredo"

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    Análise comparativa de controles positivos no processo de cicatrização de feridas cutâneas em ratos
    (Centro Universitário do Estado do Pará, 2021) Medeiros, Isabela Parente e Silva de; Carmo, Tomaz José Aquino Vasconcelos do; Chaves, Rosa Helena de Figueiredo; http://lattes.cnpq.br/8319947015729094
    Introduction: Healing is a physiological process that involves several complex mechanisms, such as the interaction between cells and inflammatory factors that aim to restore the morphological and physiological characteristics of this injured tissue. Many factors interfere in this process, such as wound hydration and use of substances with healing potential. Furthermore, both in practice and in a situation in the experimental field, it is not yet well established which is the ideal substance to assist in the healing process. Therefore, the objective of this work will be to analyze the effect on the healing of skin wounds in rats with the following positive controls: PVPI, Chlorhexidine, Saline, Hydrogel and Sunflower Oil. Objectives: To compare the effect observed in the treatment with 0.9% saline solution, Sunflower Oil, Hydrogel, Chlorhexidine and aqueous PVPI on the healing of induced skin wounds in rats. Methodology: Experimental, prospective, longitudinal, statistical-comparative study. Thirty-five rats, 5 animals destined for the pilot group and the others were randomly distributed in 5 study groups, with 5 animals each. For this, a surgical wound in the dorsal region of the animals was performed and the substances mentioned were applied daily in each experimental group, every 12 hours. On the 3rd, 7th, 14th and 21st day, macroscopic analysis of the wound was performed and after the last analysis the animals were euthanized and histological tissue processing was performed for microscopic analysis. Negative Control Group (GCN): animals that receive an application of saline solution. Sunflower Oil Group (SOG): animals that receive treatment with Sunflower Oil. Hydrogel Group (HG): animals receiving wound treatment with hydrogel. Chlorhexidine Groupp (CG): animals that were treated with chlorhexidine. Aqueous PVPI group (APG): animals that were treated with aqueous PVPI. Results: In the macroscopic evaluation, there was a decrease in the areas of mean injury over the course of days, in all groups, more pronounced from the 7th to the 14th day. On the 7th day, the OG group presented significantly larger area (mean of 560.6 mm2) when compared alone to the SF group (mean of 302.8 mm2), but the other groups did not differ significantly from each other on this day. On the 14th day, significant variation was also found (p = 0.0093, Kruskal-Wallis test), so that the LC group was statistically superior to the SF group (area means 148.4 mm2 and 66.4 mm2, respectively). There was no significant difference between other groups. In histological analysis, it was observed that the proportions of vascular proliferation did not differ significantly between the groups, with p-value not significant (p = 0.07755). There was a significant association only between the groups and the intensity of mononuclear cells (p = 0.0169) and between the groups and the intensity of collagen fibers (p = 0.0486). As for mononuclear cells, more cases were observed with marked manifestation of mononuclear cells in the HG group and discrete in the SF. There was a significant association only between the groups and the intensity of mononuclear cells (p = 0.0169) and between the groups and the intensity of collagen fibers (p = 0.0486). As for mononuclear cells, more cases were observed with marked manifestation of mononuclear cells in the HG group and discrete in the SF. Conclusion: It was concluded that there was a significant reduction within each group, in relation to the wound diameter, macroscopically more accentuated in the SF group. However, it cannot be concluded that this would be the best group for control due to more discrete collagen proliferation.
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    Avaliação de diferentes protocolos anestésicos para a realização de ovariosalpingohisterectomia em ratas
    (Centro Universitário do Estado do Pará, 2021) Franco, Pedro Pastorini; Silva Junior, Wilson Ribeiro da; Chaves, Rosa Helena de Figueiredo; Mesquita, Ellen Yasmin Eguchi; http://lattes.cnpq.br/7353490548805706; http://lattes.cnpq.br/8319947015729094
    INTRODUCTION: Experimental models are useful tools for the understanding of a range morphological changes and those related to different systems. In the context of experimental surgery, laparotomy is the procedure most used by researchers, due to the possibility of easy access to abdominal organs through surgery. In research projects, anesthesia recommended or even mandatory, depending on the procedure, therefore choosing an ideal anesthetic protocol for the procedures becomes essential. OBJECTIVES: To evaluate the effects of different anesthetic protocols in the performance of OSH in female rats by evaluating HR, RR,T°, time of induction and maintence. METHODOLOGY: Thirty-one (n=31) animals were randomly civided into 5 experimental groups. in GP: animals (n = 3) underwent OSH for technique training. In QX: animals (n = 7) anesthetized with Ketamine 80mg / Kg + Xylazine 20mg / Kg and undergoing OSH. In QXF: animals (n = 7) anesthetized with Ketamine 60 mg / kg + Xylazine 8 mg / kg + Fentanyl 0.03 mg / kg and undergoing OSH. In QM: animals (n = 7) anesthetized with Ketamine 70 mg / kg + Midazolam 5mg / kg and undergoing OSH. In QMF: animals (n = 7) anesthetized with Ketamine 60 mg kg + Midazolam 5 mg / kg + Fentanyl 0.03 mg / kg and undergoing OSH. From the induction of the animals and trought trans and postoperative period the animal were monitored regarding the above parameters. The time it took the animal to start moving and walking with coordination was also documented. After post-anesthetic monitoring, the animals were euthanized. Variables were detected in mean and standard deviation. The Kruskall-Wallis test was used (with Dunn's post-test, when necessary) and p value <0.05 was acopted. RESULT: most of the data collected was not statistically classified, but in the analysis of the means it was possible to establish that the QMF protocol was the one that kept the parameters taken close to normal for the species. CONCLUSION: Considering always keeping the HR, FR and T°C values within the normal values for the species and thus keeping the animal as close as passible to homeostasis, we can consicer that the QMF protocol was the best one that fulfilled this role in this study.